Journal: PLoS Biology
Article Title: Radiation causes tissue damage by dysregulating inflammasome–gasdermin D signaling in both host and transplanted cells
doi: 10.1371/journal.pbio.3000807
Figure Lengend Snippet: Three-month-old WT and Gsdmd -/- male mice were left untreated or subjected to 9-Gy TBI. Irradiated mice were transplanted with 10 7 bone marrow cells from 3-month-old WT or Gsdmd -/- male mice to generate WT→WT, Gsdmd -/- →WT, WT→ Gsdmd -/- , and Gsdmd -/- → Gsdmd -/- mice. Samples were harvested 3 weeks later. The femurs were analyzed by μCT. (A, C) Cross sections of 3D reconstructions. (B, D) BV/TV. The femurs were also stained for TRAP activity. (E) TRAP + cells (OCs), stained in red. (F) N.Oc/BS. Scale bar: 200 μm. The percentage of OC progenitors (“prog”) in bone marrow cells was analyzed by flow cytometry (G; the gating is shown in ). (H) H&E staining of the spleen. Scale bar: 100 μm. (I) Percentage of spleen weights. The numerical values underlying Fig 2B, 2D, 2F, 2G, 2I can be found in . Data are mean ± SEM. * P < 0.05, ** P < 0.005, *** P < 0.0005, **** P < 0.0001. μCT, micro–computed tomography; BV/TV, bone volume/total volume; GSDMD, gasdermin D; H&E, hematoxylin and eosin; N.Oc/BS, OC number/bone surface; ns, not significant; OC, osteoclast; TBI, total body irradiation; WT, wild-type.
Article Snippet: At the end of the culture period, the cells were rinsed with water and incubated with the TRAP staining solution (Sigma leukocyte acid phosphatase kit) at room temperature for 30 minutes.
Techniques: Irradiation, Staining, Activity Assay, Flow Cytometry, Micro-CT